Laser Based Point Scanning Confocal Microscope System

Trade Agreement: WTO-AGP/NAFTA/AIT/Canada FTAs with
Peru/Colombia/Panama
Tendering Procedures: All interested suppliers may submit a bid
Attachment: None
Competitive Procurement Strategy: Best Overall  Proposal
Comprehensive Land Claim Agreement: No
Nature of Requirements: 
The Bid Closing Date is extended to March 10, 2014.

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Title: Laser Based Point Scanning Confocal Microscope System

The Department of Agriculture and Agri-Food, Southern Crop
Protection and Food Research Centre (SCPFRC), London, Ontario
has a requirement for the supply and delivery of quantity one
(1), state of the art Laser Based Point Scanning Confocal
Microscope System that can accommodate a large number of diverse
applications and research needs.  The requirement also includes
the installation, training and 4 years extended warranty for the
microscope.  

Given the increased demand and diverse application strategies
targeted by numerous ongoing and anticipated research projects
at the SCPFRC, the Laser Based Point Scanning Confocal
Microscope System  will be used for high-resolution
visualization of cellular compartments and components,
macromolecular interactions, intracellular transport and
cell-to-cell movement . 

The required Laser Based Point Scanning Confocal Microscope
System must be manufactured, delivered and warrantied by one (1)
firm.

Some of the Mandatory Technical Specifications are:
1.  The system must have a modular-configuration to minimize the
potential for electrical interference or vibration and must fit
into a 7'3" x 11' room;  
2.  The system must have high resolution real-time optical
sectioning of fixed and live specimens (cells, tissues, organs)
using a laser based point scanning confocal microscope;
3.  It must have the ability to perform simultaneous imaging and
photo-stimulation experiments for photo-bleaching, diffusion and
cellular transportation studies of tagged proteins and
organelles (such as fluorescent resonance energy transfer [FRET]
imaging, fluorescence in situ hybridization [FISH] analysis,
fluorescence loss in photo-bleaching [FLIP], and fluorescence
recovery after photo-bleaching [FRAP]);
4.  The system must have Green fluorescent protein (GFP), Cyan
fluorescent protein (CFP), Yellow fluorescent protein (YFP), and
Red fluorescent protein (RFP) based multi-spectral imaging, and
advanced 3D modeling capabilities.
5. A spectral detection system with a minimum 2nm resolution
must be provided, since fluorophores with overlapping emission
spectra will be used.
6. The detection system must be capable of equalizing resolution
of multiple wavelengths in co-localization experiments;

Refer to the Request for Proposal, Annex A for  a list of
detailed  Mandatory Technical Specifications.

Delivery is required as soon as possible to SCPFRC, London,
Ontario.
Delivery Date: Above-mentioned

The Crown retains the right to negotiate with suppliers on any
procurement.

Documents may be submitted in either official language of Canada.